Synthesis and application of new solid phase techniques in quantitative proteomics using MALDI and ESI mass spectrometry
Creato da
Moschidis, Petros
Giovanni Sindona, Giovanni
Gabriele, Bartolo
Metadata
Show full item recordDescrizione
Format
/
Dottorato di Ricerca in: Methodologies for the development of molecules of pharmacological interest, Ciclo XXI,a.a. 2007-2008; Proteomics, the analysis of the protein complement of a cell or an
organism has grown rapidly as a category of the life sciences. Mass
spectrometry (MS) is one of the central detection techniques in proteome
analysis, yet it has to rely on prior sample preparation steps that reduce the
enormous complexity of the protein mixtures obtained from biological systems.
For that reason, a number of tagging (or labeling) strategies have been
developed that target specific amino acid residues or post-translational
modifications, enabling the enrichment of subfractions via affinity clean up,
resulting in the identification of an ever-increasing number of proteins. In
addition, the attachment of stable isotope-labeled tags now allow the relative
quantitation of protein levels of two samples, e.g. those representing different
cell states, which is of great significance for drug discovery and molecular
biology.
This work presents the research for new stable isotope-labeled (as well as
stable-isotope free) solid phase strategies for the identification and relative
quantitation of complex protein mixtures using MALDI and ESI-MS, MS/MS
technology.; Università della CalabriaSoggetto
Chimica; ecniche di analisi della materia; Spettrometria di massa
Relazione
CHIM/06;